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myc rab35 q67l  (Addgene inc)
90
Addgene inc myc rab35 q67l
Figure 6. Activation of <t>Rab35</t> promotes apical transport of sphingomyelin via reduction of actin cortex of apical membrane. (A) EpH4 wild-type (WT) cells and Rictor KD cells stably expressing either mScarlet only or mScarlet-tagged-Rab35 <t>Q67L</t> were treated with bSMase. bSMase was washed out and cells
Myc Rab35 Q67l, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 6. Activation of Rab35 promotes apical transport of sphingomyelin via reduction of actin cortex of apical membrane. (A) EpH4 wild-type (WT) cells and Rictor KD cells stably expressing either mScarlet only or mScarlet-tagged-Rab35 Q67L were treated with bSMase. bSMase was washed out and cells

Journal: The Journal of cell biology

Article Title: mTORC2 suppresses cell death induced by hypo-osmotic stress by promoting sphingomyelin transport.

doi: 10.1083/jcb.202106160

Figure Lengend Snippet: Figure 6. Activation of Rab35 promotes apical transport of sphingomyelin via reduction of actin cortex of apical membrane. (A) EpH4 wild-type (WT) cells and Rictor KD cells stably expressing either mScarlet only or mScarlet-tagged-Rab35 Q67L were treated with bSMase. bSMase was washed out and cells

Article Snippet: The amplified fragment supplemented with mScarlet was sub-cloned into the pLenti CMV Neo DEST vector (Plasmid #17392 from Addgene). pmScarlet-H_Giantin_C1 (Plasmid #85049), Myc-Rab35 WT (Plasmid #47433), Myc-Rab35 Q67L (Plasmid #405909), and pcDNA3-FlipGFP (Casp3 cleavage seq) T2A mCherry (Plasmid #124428) were obtained from Addgene.

Techniques: Activation Assay, Membrane, Stable Transfection, Expressing